Pluripotent Stem Cells Research

Proteomic and functional comparison between human induced and embryonic stem cells

This study compares the proteomes of human induced pluripotent stem cells (hiPSCs) and embryonic stem cells (hESCs), finding quantitative differences in protein expression affecting growth, metabolism, and secreted factors.

Cryopreservation of neuroectoderm on a pillar plate and in situ differentiation into human brain organoids

This paper develops a cryopreservation technique for human brain organoids using a pillar plate platform, showing that smaller organoids and specific cryoprotective agents improve viability and differentiation.

PI3K/AKT signalling orchestrates ICM maturation and proper epiblast and primitive endoderm specification

This study identifies PI3K/AKT signaling as an upstream regulator that enables ICM formation and specifies both epiblast and primitive endoderm lineages in mouse embryos.

TASOR expression in naive embryonic stem cells safeguards their developmental potential

This study identifies TASOR's role in maintaining developmental potential during the transition from naive to primed pluripotency by preventing L1 transposable element activation and subsequent cell death.

Inhibition of Proteasome Activity Facilitates Definitive Endodermal Specification of pluripotent Stem Cells by influencing YAP signaling

This study shows that inhibiting proteasome activity enhances definitive endodermal specification in pluripotent stem cells through YAP signaling.

Parallel genome-scale CRISPR-Cas9 screens uncouple human pluripotent stem cell identity versus fitness

The paper uses CRISPR-Cas9 screens to identify genes affecting stem cell fitness versus pluripotency identity, finding distinct and overlapping roles for chromatin and metabolic regulators.

Assembloid model to study loop circuits of the human nervous system

The paper develops an assembloid model of the human cortico-striatal-thalamic-cortical circuit to study its function and disease-related disruptions.

Fetal Liver-like Organoids Recapitulate Blood-Liver Niche Development and Multipotent Hematopoiesis from Human Pluripotent Stem Cells

Human sensory-like neuron cultivation - an optimized protocol

This study optimizes a protocol for culturing induced sensory-like neurons (iSN) by reducing non-iSN cells while maintaining iSN viability and functionality.

A repository of Ogden syndrome (NAA10) and NAA15 patient derived iPSC lines and isogenic pairs by X-chromosome screening and genome-editing.

The paper describes the generation and characterization of patient-derived iPSC lines and isogenic pairs for Ogden syndrome, including CRISPR corrections and X-chromosome screening.

A longevity-specific bank of induced pluripotent stem cells from centenarians and their offspring

The paper establishes a resource of iPSCs from centenarians and their offspring to study human longevity and resilience.

GABAergic neuron dysregulation in a human neurodevelopmental model for major psychiatric disorders

This study investigates GABAergic neuron dysfunction in psychiatric disorder models, finding mutation-dependent synaptic deficits and shared gene expression changes in DISC1 mutant neurons.

Transient Notch Activation Converts Pluripotent Stem Cell-Derived Cardiomyocytes towards a Purkinje Fiber Fate

The paper shows that transient Notch activation converts human pluripotent stem cell-derived cardiomyocytes into Purkinje fiber-like cells with altered electrophysiology and gene expression.

LINE1 promotes nuclear compartmentalization to repress the 8-cell state in embryonic stem cells

This paper shows that LINE1 RNA is essential for maintaining hESCs by preventing the 8C state through nuclear compartmentalization and PRC2-mediated repression.

FoxO transcription factors actuate the formative pluripotency specific gene expression programme

This study identifies FoxO transcription factors as essential for transitioning from naive to formative pluripotency by regulating enhancer activity through AKT signaling.

Pcbp1 constrains Oct4 expression in the context of pluripotency

This study shows Pcbp1 regulates Oct4 expression during differentiation, with loss of Pcbp1 causing premature differentiation and increased PrE specification.

Generating human parvalbumin interneurons through 3D glia reprogramming

This paper describes a method to generate human parvalbumin interneurons from glial precursor cells using direct reprogramming and identifies a distinct transcriptional profile and reprogramming trajectory.

A community-oriented, data-driven resource to improve protocol design for cardiac modelling from human pluripotent stem cells

The paper develops a resource to improve cardiac modelling protocols using hPSC-CMs by analyzing decades of research data and providing tools for protocol benchmarking.

LMNA-Related Dilated Cardiomyopathy: Single-Cell Transcriptomics during Patient-derived iPSC Differentiation Support Cell type and Lineage-specific Dysregulation of Gene Expression and Development for Cardiomyocytes and Epicardium-Derived Cells with Lamin A/C Haploinsufficiency

This study uses single-cell RNA sequencing of patient-derived iPSCs to identify gene expression and pathway dysregulation in LMNA haploinsufficiency affecting cardiomyocytes and epicardium-derived cells.

Single-cell multiome uncovers differences in glycogen metabolism underlying species-specific speed of development

The paper identifies glycogen metabolism differences in pluripotent cells across species that influence neural differentiation speed.

An Efficient Direct Conversion Strategy to Generate Functional Astrocytes from Human Adult Fibroblasts

This study develops a method to efficiently convert adult human fibroblasts into functional astrocytes using small molecules, achieving high conversion efficiency and astrocyte-like characteristics.

Rapid specification of human pluripotent stem cells to functional astrocytes

This study develops a method to differentiate human pluripotent stem cells into functional astrocytes in 28 days, validated by marker expression and network activity support.

Transcription factor-based transdifferentiation of human embryonic to trophoblast stem cells

The paper identifies transcription factors that can convert human embryonic stem cells into trophoblast stem cells, revealing their role in early placental development.

Posterior specification of multi-lineage axial assembloids from human pluripotent stem cells.

This paper describes a protocol to generate human posterior axial assembloids from pluripotent stem cells, demonstrating multi-lineage differentiation and tissue complexity.

Epigenomic anomalies in induced pluripotent stem cells from Alzheimer disease cases

This study identifies epigenomic anomalies in iPSCs derived from Alzheimer's disease patients that affect neural differentiation and amyloid/Tau pathology.

Cross-site reproducibility of human cortical organoids reveals consistent cell type composition and architecture

This study shows that human cortical organoids generated across multiple sites using a harmonized protocol consistently produce similar cell types and structures, though with some site-specific differences in size and stress markers.

De novo cancer mutations frequently associate with recurrent chromosomal abnormalities during long-term human pluripotent stem cell culture

This study tracks genetic changes in human pluripotent stem cells over time, finding that recurrent chromosomal gains drive the accumulation of other mutations.

The level of HAND1 controls the specification of multipotent cardiac and extraembryonic progenitors

This study identifies HAND1's concentration-dependent role in specifying cardiac and extraembryonic progenitors from human pluripotent stem cells.

Centimeter-scale, physiologically relevant intestinal organoids generated entirely from pluripotent stem cells

This study generates centimeter-scale, physiologically relevant intestinal organoids from iPSCs without Matrigel, showing mature structures and immune-related cell types.

The Interferon γ Pathway Enhances Pluripotency and X-Chromosome Reactivation in iPSC Reprogramming

This study identifies the IFNγ pathway's role in accelerating iPSC reprogramming and X-chromosome reactivation through STAT3 signaling and TET-mediated demethylation.