Hematopoietic Stem Cell Transplantation

Hematopoietic lineages diverge within the stem cell compartment

The paper uses fate-mapping, single-cell RNA-sequencing, and computational methods to show that major hematopoietic lineages diverge within HSCs, identifying a self-renewing HSC subpopulation and a direct thrombopoiesis pathway.

Mesoderm-Derived PDGFRA+ Cells Regulate the Emergence of Hematopoietic Stem Cells in the Dorsal Aorta

The paper shows that mesoderm-derived PDGFRA+ stromal cells in the AGM region support endothelial-to-hematopoietic transition and LT-HSC generation.

In vivo CRISPR screening identifies SAGA complex members as key regulators of hematopoiesis

This study uses in vivo CRISPR screening to identify SAGA complex members as key regulators of hematopoiesis, showing their loss impairs blood production and activates interferon pathways.

Single-cell transcriptome analysis of embryonic and adult endothelial cells allows to rank the hemogenic potential of post-natal endothelium

The paper uses single-cell transcriptomics to compare embryonic and adult endothelial cells, identifying adult endothelial cell types with high potential for reprogramming into hematopoietic stem cells.

Identification of fetal liver stromal subsets in spectral cytometry using the parameter autofluorescence

The paper identifies fetal liver stromal subsets using spectral cytometry and autofluorescence, finding two distinct autofluorescent signals corresponding to hepatoblasts/cholangiocytes and stellate cells.

A specialized bone marrow microenvironment for fetal haematopoiesis

The paper identifies a specialized fetal bone marrow niche regulated by arterial endothelial cells and Wnt2 signaling that supports hematopoietic stem and progenitor cell expansion.

Distinctive Transcriptional and Microbial Signature in Cutaneous Acute Graft-vs-Host-Disease

The paper identifies a distinctive transcriptional and microbial signature in cutaneous acute graft-vs-host disease through RNA-sequencing and microbiome analysis of patient samples.

DE-NOVO HEMATOPOIESIS FROM THE FETAL LUNG

The paper shows that fetal lung contains hemogenic endothelial cells capable of undergoing endothelial to hematopoietic transition to generate de-novo hematopoietic stem and progenitor cells.

MyD88-dependent TLR signaling oppositely regulates hematopoietic progenitor and stem cell formation in the embryo

The paper shows that MyD88-dependent TLR signaling in endothelial cells reduces hematopoietic stem cells (HSCs) while increasing committed progenitors and intra-arterial hematopoietic cluster cells by promoting their proliferation.

Genome-wide association study on 13,167 individuals identifies regulators of hematopoietic stem and progenitor cell levels in human blood

A genome-wide association study identifies DNA variants influencing HSPC levels in human blood, with PPM1H as a novel regulator affecting stem cell subpopulations.

Developmental maturation of the hematopoietic system controlled by a Lin28b-let-7-PRC1 axis

The paper identifies a Lin28b-let-7-PRC1 axis that regulates hematopoietic maturation across development, with Cbx2 playing a key role in controlling developmental timing of hematopoietic transcription factors.

Lyl-1 regulates primitive macrophages and microglia development

This study identifies Lyl-1 as a critical regulator of primitive macrophage and microglia development, showing its role in progenitor emergence and differentiation.

HSC-independent definitive hematopoietic cells persist into adult life

The paper shows that HSC-independent hematopoietic progenitors persist into adulthood, challenging the HSC-centric model of blood cell development.

Niche signals regulate continuous transcriptional states in hematopoietic stem cells

The paper identifies six HSC states and shows niche perturbations induce rapid transitions between them, revealing HSC-specific molecular signatures and chromatin features related to regenerative potential.

Early epigenetic influence on EGR1, KLF2 and KLF4 transcriptional network associates with alteration of HSCs quiescence

The paper links DNA methylation changes in HSCs from large for gestational age neonates to altered quiescence via transcriptional network disruption.

A Cdc42-Borg4-Septin 7 axis regulates HSCs polarity and function

This study identifies a Cdc42-Borg4-Sep7 axis that regulates HSC polarity and function by controlling the polar distribution of these proteins and affecting engraftment potential and LMPP frequency.

Identifying a novel role for the master regulator Tal1 in the Endothelial to Hematopoietic Transition

The paper investigates the role of Tal1, Lmo2, and Lyl1 in the Endothelial to Hematopoietic Transition using a mouse embryonic-stem cell model and multi-omics approaches, finding that Tal1 expression in endothelial cells is crucial for the transition.

Intrinsic and extrinsic regulation of human fetal bone marrow haematopoiesis and perturbations in Down syndrome

This study uses single-cell RNA-sequencing to analyze fetal bone marrow development and identifies disruptions in hematopoiesis in Down syndrome.

Endothelial-specific Gata3 expression is required for haematopoietic stem cell generation

This study shows that endothelial-specific Gata3 expression is required for the maturation of haemogenic endothelial cells into haematopoietic stem cells during the endothelial-to-haematopoietic transition.

p57Kip2 regulates embryonic haematopoietic stem cell numbers by controlling the size of the sympathoadrenal progenitor pool

The paper shows p57Kip2 limits HSC numbers by restricting SNS progenitor size and catecholamine secretion in the AGM region via β2-adrenergic signaling.

HLF Expression Defines the Human Hematopoietic Stem Cell State

This study identifies HLF as a specific marker for human hematopoietic stem cells and develops a genomic reporter system to track its expression.

Redundant mechanisms driven independently by RUNX1 and GATA2 for hematopoietic development

The paper identifies RUNX1-independent mechanisms for hematopoietic stem cell generation and shows that GATA2 and RUNX1 functionally complement each other in hematopoiesis.

Definitive Hematopoietic Stem Cells Minimally Contribute to Embryonic Hematopoiesis

This study shows nascent hematopoietic stem cells (HSCs) self-renew but have delayed differentiation during embryonic hematopoiesis, with HSC-independent progenitors driving early blood development.

Multipotent progenitors and hematopoietic stem cells arise independently during the endothelial to hematopoietic transition in the early mouse embryo

The paper shows that multipotent progenitors and HSC arise from distinct HE populations during mouse embryogenesis, identified through clonal assays and single-cell transcriptomics.

Hematopoiesis at single cell resolution spanning human development and maturation

The paper uses single cell RNA-seq to study human hematopoietic stem and progenitor cells (HSPCs) across development and aging, revealing age-related transcriptional changes and differentiation trajectories.

DDX41 is needed for pre-and post-natal hematopoietic stem cell differentiation in mice

This study shows that DDX41 is essential for hematopoietic stem cell differentiation in mice, particularly affecting myeloid progenitor development and gene expression programs.

An abundant myeloid progenitor shapes neonatal hematopoiesis of naked mole-rats

The paper identifies a unique myeloid progenitor cell type, TC90, specific to neonatal naked mole-rats that supports innate immunity during early development.

KIT is required for fetal liver erythropoiesis but dispensable for angiogenesis

The paper shows KIT is required for fetal liver erythropoiesis but not for yolk sac endoHT or brain angiogenesis.

Detection of hematopoietic stem cell transcriptome in human fetal kidneys and kidney organoids derived from human induced pluripotent stem cells (iPSC)

The paper identifies cells with hematopoietic stem cell (HSC) transcriptome features in human fetal kidneys and iPSC-derived kidney organoids.

Developmental stage- and site-specific transitions in lineage specification and gene regulatory networks in human hematopoietic stem and progenitor cells

This study identifies site- and stage-specific transitions in gene regulatory networks and lineage specification in human hematopoietic stem and progenitor cells across developmental stages.