Pluripotent Stem Cells Research

Paradoxical effects of Zim3, a CRISPRi effector, on human iPSC-cardiomyocyte electrophysiology

The paper shows that Zim3, used as a CRISPRi effector without guide RNAs, upregulates cardiac ion channel genes in iPSC-CMs, leading to a more mature phenotype.

Application of Elevated Atmospheric Pressure and Hypoxia Enhance Pluripotency and Stem Cell Differentiation

This study shows that elevated atmospheric pressure and hypoxia enhance reprogramming of fibroblasts to iPSCs and promote differentiation into neural and hematopoietic lineages.

Generation and benchmarking of a collection of hiPSC lines from Schizophrenia Patients with Diverse Clinical Profiles

The paper generates and validates a collection of hiPSC lines from Schizophrenia patients with diverse clinical profiles to model disease-specific neural phenotypes and develop targeted therapies.

Histone demethylase KDM5 regulates cardiomyocyte maturation by promoting fatty acid oxidation, oxidative phosphorylation, and myofibrillar organization

This study shows that KDM5 inhibition promotes iPSC-CM maturation by enhancing fatty acid oxidation, oxidative phosphorylation, and myofibrillar organization through epigenetic regulation of key genes.

Transcription Factor Co-Expression Mediates Lineage Priming for Embryonic and Extra-Embryonic Differentiation

The paper identifies ESCs co-expressing GATA6 and SOX2, showing their cooperative DNA binding in enhancers and role in lineage priming for embryonic and extra-embryonic differentiation.

Amino acid intake strategies define pluripotent cell states

This study shows that mouse embryonic stem cells (ESCs) can proliferate without essential amino acids by taking up and digesting exogenous protein, mimicking pre-implantation embryos.

Combined small molecule treatment accelerates timing of maturation in human pluripotent stem cell-derived neurons

This study identifies a drug cocktail that accelerates maturation of hPSC-derived neurons and other cell types through pharmacological intervention.

DNA methylation restricts coordinated germline and neural fates in embryonic stem cell differentiation

This study shows that DNA methylation is critical for restricting neural differentiation but not germline fate in ESCs, with TKO ESCs capable of germline specification.

In vitro generation of megakaryocytes from engineered mouse embryonic stem cells

This paper describes a method to differentiate mouse embryonic stem cells into megakaryocytes using synthetic gene circuits and specific feeder cell cultures.

Generation of cultured beef from bovine embryonic stem cells

This study generates cultured beef from bovine embryonic stem cells by establishing a pluripotent cell line, developing differentiation protocols, and creating a plant-cell hybrid scaffold.

Incomplete Reprogramming of DNA Replication Timing in Induced Pluripotent Stem Cells

The paper examines DNA replication timing in iPSCs, finding that some exhibit delayed replication at heterochromatic regions, which persists through differentiation and may affect their quality.

DMSO derives Trophectoderm and Clonal Blastoid from Single Human Pluripotent Stem Cell

This study shows that DMSO induces trophectoderm differentiation and blastoid formation from single human pluripotent stem cells without additional perturbations.

The Nucleosome Remodelling and Deacetylation complex coordinates the transcriptional response to lineage commitment in pluripotent cells

This study shows NuRD complex coordinates gene expression during lineage commitment by reducing transcriptional noise, priming genes for activation, and maintaining stable states.

In vitro differentiation of mouse pluripotent stem cells into glucocorticoid-producing adrenocortical cells

This study develops a step-wise in vitro differentiation system from mouse embryonic stem cells into glucocorticoid-producing adrenocortical cells using fibronectin and cAMP activators.

Enhancing Sox/Oct cooperativity induces higher-grade developmental reset

This study identifies a Sox2 mutation that enhances Sox/Oct cooperativity, improving iPSC reprogramming efficiency and enabling reprogramming of aged cells across species.

HLA-Based Banking of Human Induced Pluripotent Stem Cells in Saudi Arabia

This study establishes an HLA-based iPSC banking system for the Saudi population, demonstrating that a small number of iPSC lines can cover a significant portion of the population's HLA haplotypes.

Modelling Human Post-Implantation Development via Extra-Embryonic Niche Engineering

The paper introduces iDiscoid, a human stem cell model of post-implantation development that recapitulates key morphogenetic features and offers a platform for studying early human development.

CRISPR loss of function screening to identify genes involved in human primordial germ cell-like cells development

This study uses CRISPR screening to identify genes critical for human primordial germ cell-like cell development, finding TCL1A regulates AKT-mTOR signaling and protein synthesis.

TGF-beta dynamically controls epithelial identity in a 3D model of human epiblast

This study develops a 3D model of human pluripotent epiblast formation and shows that TGF-beta dynamically maintains epithelial identity, independent of canonical pluripotency markers.

Enhanced Production of Mesencephalic Dopaminergic Neurons from Lineage-Restricted Human Undifferentiated Stem Cells

This study genetically modifies human pluripotent stem cells to generate lineage-restricted undifferentiated stem cells that efficiently produce mesencephalic dopaminergic neurons for potential clinical applications.

Osmolar modulation drives reversible cell cycle exit and human pluripotent cell differentiation via NF-κB and WNT signaling

This study shows that hyperosmolar conditions induce cell cycle exit and differentiation in pluripotent cells through NF-κB and WNT signaling pathways.

Dissecting embryonic and extra-embryonic lineage crosstalk with stem cell co-culture

This study establishes co-cultures of embryonic and extraembryonic stem cells to study lineage crosstalk during early mammalian development, revealing growth inhibition of pluripotent cells by extraembryonic endoderm cells.

Human naive stem cell models reveal the role of FGF signalling in hypoblast specification in the human embryo

This study uses human naive PSC models to show FGF signaling is critical for hypoblast specification, revealing conservation with mouse lineage specification.

Synthetic genetic circuits to uncover and enforce the OCT4 trajectories of successful reprogramming of human fibroblasts

The paper identifies OCT4 trajectories critical for reprogramming human fibroblasts to iPSCs and develops a synthetic genetic circuit to enforce these trajectories, improving reprogramming efficiency.

In vivo reprogramming leads to premature death due to hepatic and intestinal failure.

This study shows that continuous in vivo reprogramming with OSKM causes hepatic and intestinal failure, leading to premature death, but a new mouse strain reduces these effects.

Cadherins modulate the self-organizing potential of gastruloids

This study identifies a Cadherin switch mechanism that regulates self-organization in gastruloids by linking pluripotency exit and cell differentiation.

Integration of Xeno-Free Single-cell Cloning in CRISPR-mediated DNA Editing of Human iPSCs Improves Homogeneity and Methodological Efficiency of Cellular Disease Modelling

This paper introduces a xeno-free single-cell cloning method for CRISPR-edited human iPSCs, achieving high clonal survival and editing efficiency for improved disease modeling.

Deciphering the mRNA-lncRNA-miRNA interaction landscape in human pluripotency

The paper reconstructs the mRNA-lncRNA-miRNA interaction network in human pluripotency, identifies an alternative circuit without NANOG, SOX2, and POU5F1, and discovers DANT1 as a key ceRNA.

ERas-Null Mice Generated Directly from Embryonic Stem Cells in a Lipid-rich Medium Enable the Discovery of A Novel Role in Craniofacial Development

The paper demonstrates that lipid-rich 2iLA medium enables the generation of ERas-null all-ESC mice, revealing a novel role for ERas in craniofacial development.

Multi-omics characterization of partial chemical reprogramming reveals evidence of cell rejuvenation

This study uses multi-omics analysis to show that chemical reprogramming reduces biological age in mouse fibroblasts by altering metabolism and mitochondrial function.