Pluripotent Stem Cells Research

Transgene directed induction of a stem cell-derived human embryo model

This paper introduces a stem cell-derived human embryo model that mimics post-implantation development and identifies SOX17's inhibitory role in anterior hypoblast-like cell specification.

A Highly Efficient Aorta-Gonad-Mesonephros-Like Definitive Hemogenic Endothelium From Human Pluripotent Stem Cells

This paper describes a method to efficiently generate definitive hemogenic endothelium from human pluripotent stem cells, which can differentiate into hematopoietic progenitors and stem cells with multilineage potential.

High density SNP array and reanalysis of genome sequencing uncovers CNVs associated with neurodevelopmental disorders in KOLF2.1J iPSCs

The paper identifies CNVs in KOLF2.1J iPSCs linked to neurological disorders, showing they arose in vitro and affect gene expression in neural cells.

Directed differentiation of mouse pluripotent stem cells into functional lung-specific mesenchyme

This study generates lung-specific mesenchyme from mouse iPSCs, identifies key signaling pathways, and demonstrates functional interactions with lung epithelial progenitors.

Village in a dish: a model system for population-scale hiPSC studies

The paper demonstrates that village cultures of hiPSCs maintain consistent transcriptional profiles across uni- and village-cultured samples, enabling population-scale studies.

Combined Lineage Tracing and scRNA-seq Reveals Unexpected First Heart Field Predominance of Human iPSC Differentiation

The paper uses lineage tracing and scRNA-seq to show that human iPSC differentiation predominantly generates left ventricular cardiomyocytes from the first heart field.

Robust induction of functional astrocytes using NGN2 expression in human pluripotent stem cells

This study develops a rapid, scalable method to generate functional human astrocytes from pluripotent stem cells using NGN2 induction, showing high homogeneity and disease relevance.

Enhancer status in the primitive endoderm supports unrestricted lineage plasticity in regulative development

The paper shows that primitive endoderm cells can regenerate a complete blastocyst and develop to term, indicating their lineage plasticity and potential for multi-lineage differentiation.

Ampk activation by glycogen expenditure primes the exit of naive pluripotency

The paper shows that glycogen depletion activates Ampk in naive ESCs, promoting their transition to a primed state by reducing fatty acid synthesis and altering pluripotency markers.

The emergence of human gastrulation upon in vitro attachment

This study shows human blastoids undergo gastrulation upon in vitro attachment, replicating the transcriptomic signature of natural human gastrula and suggesting an earlier onset of gastrulation than previously thought.

Cardiopharyngeal Mesoderm specification into cardiac and skeletal muscle lineages in gastruloids.

This study shows that mouse embryonic stem cell-derived gastruloids can differentiate into both cardiac and skeletal muscle lineages, revealing insights into cardiopharyngeal mesoderm specification.

Tbx5 overexpression in embryoid bodies increases TAK1 expression but does not enhance the differentiation of sinoatrial node cardiomyocytes.

This study shows Tbx5 overexpression increases TAK1 but does not enhance SAN differentiation, while TAK1 inhibition promotes SAN gene expression in cardiomyocytes.

Symmetry breaking of hPSCs in micropattern generates a polarized spinal cord-like organoid (pSCO) with dorsoventral organization

This study generates a polarized spinal cord-like organoid with dorsoventral organization using geometrically confined hPSC cultures and shows that BMP and Shh signaling control DV patterning.

Lactate and Immunomagnetic-purified iPSC-derived Cardiomyocytes Generate Comparable Engineered Cardiac Tissue Constructs

This study compares the functional and molecular properties of engineered cardiac tissue constructs made from hiPSC-CMs purified by lactate or MACS methods, finding no significant differences.

Deriving Schwann Cells from hPSCs Enables Disease Modeling and Drug Discovery for Diabetic Peripheral Neuropathy

This study derives Schwann cells from human pluripotent stem cells to model diabetic peripheral neuropathy and identify a drug candidate that rescues sensory function and prevents myelin damage.

Bovine blastocyst like structures derived from stem cell cultures

The paper develops a method to generate bovine blastocyst-like structures (blastoids) from stem cells, which mimic blastocysts in morphology and transcriptomes.

Establishment of Bovine Trophoblast Stem Cells

This study uses a chemical cocktail to derive and culture bovine trophoblast stem cells, which retain developmental potency.

Molecular and functional characterization of different BrainSphere models for use in neurotoxicity testing on microelectrode arrays

The paper characterizes different BrainSphere models for neurotoxicity testing using microelectrode arrays, finding that media choice affects differentiation patterns and functional activity.

Derivation of trophoblast stem cells unveils unrestrained potential of mouse ESCs and epiblast

This study derives trophoblast stem cells from mouse ESCs, showing they can contribute to the trophoectoderm and placenta, challenging the notion of restricted developmental potential.

An efficient induction method for human spinal lower motor neurons and high-throughput image analysis at the single cell level

This paper introduces a new method to efficiently induce human spinal lower motor neurons from iPSCs and presents a single-cell evaluation system using time-lapse microscopy and machine learning.

Interspecies blastocyst complementation generates functional rat cell-derived forebrain tissues in mice

This study uses interspecies blastocyst complementation to generate functional rat-derived forebrain tissues in mice, revealing developmental and xenogeneic barriers.

Efficient self-organization of blastoids solely from mouse ESCs is facilitated by transient reactivation of 2C gene network

This study shows mouse ESCs can efficiently self-organize into blastoids resembling mouse blastocysts through transient reactivation of the 2C gene network.

Single-cell analysis of bidirectional reprogramming between early embryonic states reveals mechanisms of differential lineage plasticities

The paper uses single-cell RNA and ATAC-seq to study bidirectional reprogramming between embryonic states, revealing differences in lineage plasticity.

Laminin switches terminal differentiation fate of human trophoblast stem cells under chemically defined culture conditions.

This paper establishes a chemically defined culture system for differentiating human trophoblast stem cells into extravillous trophoblast and syncytiotrophoblast lineages, showing that laminin-1 switches differentiation fate.

ZFP281 coordinates DNMT3 and TET1 for transcriptional and epigenetic control in pluripotent state transitions

This study identifies ZFP281 as a coordinator of DNMT3A/3B and TET1 to regulate DNA methylation and gene expression during pluripotent stem cell state transitions.

Genetic dissection of the pluripotent proteome through multi-omics data integration

This study integrates multi-omics data from mouse embryonic stem cells to identify genetic drivers of proteome variability and uncover regulatory mechanisms in pluripotency.

Effects of somatic mutations on cellular differentiation in iPSC models of neurodevelopment

This study investigates how somatic mutations affect dopaminergic neuron differentiation in iPSCs, finding that deleterious mutations in key genes like BCOR are linked to differentiation failure and altered proliferation.

A Flexible Transgene Integration Landing-Pad Toolkit in Human Induced Pluripotent Stem Cells Enables Facile Cellular Engineering, Gene Zygosity Control, and Parallel Transgene Integration

This paper introduces a toolkit for transgene integration in hiPSCs using a safe-harbour locus, enabling controlled transgene configurations and efficient cellular engineering.

The Wnt/TCF7L1 transcriptional repressor axis drives primitive endoderm formation by antagonizing naive and formative pluripotency

This study shows that Wnt/TCF7L1 repression promotes primitive endoderm differentiation in mESCs by inhibiting naive and formative pluripotency factors.

Impact of in vivo cyclic reprogramming on the choroid plexus

This study investigates the effects of cyclic in vivo OSKM reprogramming on the choroid plexus, finding limited transcriptomic changes but increased NKCC1 expression and minor morphological alterations.